FIG 1.
Effect of BXA treatment on the replication and transmission of BXA-Sen BR/08 virus in ferrets. (A) Donor ferrets (n = 3) were inoculated with 105 TCID50/ferret of recombinant influenza BR/08-I38 virus. At 1 dpi, donors were injected subcutaneously with a single dose of BXA at 8 mg/kg or 0.5% methylcellulose, and naive DC and AC ferrets were individually paired with each donor immediately after antiviral treatment. Virus titers (log10TCID50/ml; limit of detection: 0.75 log10TCID50/ml, horizontal dotted lines) in individual ferrets were assayed using nasal washes collected at the indicated time points after donor inoculation or contact exposure. The mean viral titers in vehicle-treated and BXA-treated donors were compared at 2 dpi (#, P = 0.03) and 4 dpi (†, P = 0.05), using two-way ANOVA, followed by Šidák’s multiple-comparison post hoc test. (B) The AUCs for virus shedding from ferrets are presented as individual animal shedding curve values, which were calculated in GraphPad Prism 8.0. P values were calculated by two-way ANOVA with Šidák’s multiple-comparison post hoc test; the associated bars are color coded to indicate the groups being compared. *, P < 0.05; **, P < 0.01.