FIG 3.
Effect of BXA treatment on the replication and transmission of BXA-Res BR/08 virus in ferrets. (A) Donor ferrets (n = 3) were inoculated with recombinant influenza BR/08-I38T virus at 105 TCID50/ferret. At 1 dpi, donors were injected subcutaneously with a single dose of BXA at 8 mg/kg or 0.5% methylcellulose, and naive DC ferrets (n = 3) and AC animals (n = 3) were individually paired with each donor immediately after antiviral treatment. Virus titers (log10TCID50/ml; limit of detection: 0.75 log10TCID50/ml, horizontal dotted lines) in individual ferrets were assayed using nasal washes collected at the indicated time points after donor inoculation or contact exposure. (B) The AUCs for virus shedding from ferrets are presented as individual animal shedding curve values, which were calculated in GraphPad Prism 8.0. P values were calculated using two-way ANOVA with Šidák’s multiple-comparison post hoc test; the associated bars are color coded to indicate the groups being compared.