FIG 7.

Replication and transmission of BXA-Sen PH/13-I38 and BXA-Res PH/13-I38T viruses in a competitive fitness ferret model. (A) Donor ferrets (n = 3) were coinoculated with a mixture (in a 1:1 ratio) of the recombinant BXA-Sen PH/13-I38 virus (5 × 10⁴ TCID₅₀) and the BXA-Res PH/13-I38T virus (5 × 10⁴ TCID₅₀) in 0.5 ml of PBS. At 1 dpi, donors were injected subcutaneously with a single dose of BXA at 8 mg/kg or 0.5% methylcellulose, and naive DC and AC ferrets were individually paired with each donor immediately after antiviral treatment. Virus titers (log₁₀TCID₅₀/ml; limit of detection: 0.75 log₁₀TCID₅₀/ml, horizontal dotted lines) in individual ferrets were assayed using nasal washes collected at the indicated time points after donor inoculation or contact exposure. (B) The AUCs for virus shedding from ferrets are presented as individual animal shedding curve values, which were calculated in GraphPad Prism 8.0. P values were calculated using two-way ANOVA with Šidák’s multiple-comparison post hoc test; the associated bars are color coded to indicate the groups being compared. **, P < 0.01.