TABLE 3.

Overview of eis C-14T isolates with eis LoF mutations and corresponding MICs for KAN and AMK^a

Isolate ID	Present in 2.2.1.1.1.3.i3 cluster	rrs A1401G	rrs C1402T	rrs G1484T	eis C-14T	Eis LOF mutation	MIC (mg/liter) of:
							KAN	AMK
IT184	No	A	C	G	Yes	No	8	0.5
IT123	No	A	C	G	Yes	No	16	0.5
655-19	No	A	C	G	Yes	No	16	1
IT233	Yes	A	C	G	Yes	No	16	1
IT952	No	A	C	G	Yes	No	16	2
IT524	No	A	C	G	Yes	No	>16	2
IT77	Yes	A	C	G	Yes	No	>16	2
622-19	Yes	A	C	G	Yes	Yes (279delCGGCGATGCGT)	≤1	≤0.25
IT1070	Yes	A	C	G	Yes	Yes (448delA)	≤1	≤0.25
IT947	Yes	A	C	G	Yes	Yes (627insC)	≤1	≤0.25
168-19	No	A	C	G	Yes	Yes (400insG)	≤1	≤0.25
IT634	Yes	A	C	G	Yes	Yes (V1V)	2	≤0.25
SAMN02419559b	Yes	A	C	G	Yes	Yes (V1V)
SAMN02419535b	Yes	A	C	G	Yes	Yes (V1V)
SAMN02419543b	Yes	A	C	G	Yes	Yes (V1V)
SAMN02419586b	Yes	A	C	G	Yes	Yes (V1V)
SAMN07236283b	No	A	C	G	Yes	Yes (V1V)
SAMEA1016073	Yes	A	C	G	Yes	Yes (448delA)
SAMEA1403685	Yes	A	C	G	Yes	Yes (448delA)
SAMEA1403638	Yes	A	C	G	Yes	Yes (448delA)
SAMN02584676b	Yes	A	C	G	Yes	Yes (448delA)
SAMN04633319b	Yes	A	C	G	Yes	Yes (448delA)
SAMN08376196b	No	A	C	G	Yes	Yes (W13*)
SAMN08709032b	No	A	C	G	Yes	Yes (W13*)
SAMN06210015b	No	A	C	G	Yes	Yes (16insC)
Peru2946	No	A	C	G	Yes	Yes (486insCT)
Peru3354	No	A	C	G	Yes	Yes (486insCT)
SAMN02584612b	No	A	C	G	Yes	Yes (15insC)
SAMN07956543b	Yes	A	C	G	Yes	Yes (448delA and 473insT)

^aAll 29 double mutants lacked the three classical AG resistance mutations in rrs (A, C, and G are the wild-type alleles for the A1401G, C1402T, and G1484T resistance mutations, respectively). Isolates that are part of the 2.2.1.1.1.3.i3 cluster are shown in Fig. 3. MICs were measured using either the UKMYC5 or UKMYC6 broth microdilution plates by Thermo Fisher Scientific (54). The provisional CCs for KAN and AMK for these plates are 4 and 1 mg/liter, respectively. Unlike for KAN, eis C-14T only has a modest effect on the MIC of AMK (i.e., the MIC distribution of this mutation spans the CC when the efflux pump is active, which is in line with data from other media) (9, 14–16, 68). Consequently, KAN is a more sensitive agent to detect an inactive efflux pump than AMK. More details can be found in Table S6 in the supplemental material.

^bBioSample accession numbers are shown.