FIG 2.

In vitro formation of AT-9010 in human peripheral blood mononuclear cells. PBMCs were incubated with 10 μM AT-281 for 6 h. The prodrug was then removed, and the cells were rinsed and incubated for another 28 h (washout phase). At each time point, cells (n = 3) were collected in ice-cold 60% MeOH and analyzed for AT-9010, the active intracellular TP, using an LC-MS/MS assay as described in Materials and Methods. Data are expressed as means ± standard deviations (SDs).