FIG 9.

Micafungin induced the PTV in a manner dependent on ER Ca²⁺ stores. (A) The exposure of mouse tracheae to low-dose caffeine (15 mM) induced a sustained rise in the PTV that was not further increased by micafungin application. (B) A high caffeine concentration (30 mM) did not trigger a PTV increase, and subsequent application of micafungin also did not provoke a rise in the PTV. (C) Ryanodine at a high concentration (40 μM) prevented the micafungin-induced rise in the PTV. (D) A low caffeine concentration (15 mM) evoked a rise in the PTV that was not further enhanced in the presence of micafungin. A high caffeine concentration (30 mM) did not accelerate the PTV, and subsequent application of micafungin did not change the PTV. A high ryanodine concentration did not evoke an increase in the PTV, and successive micafungin application did not increase the PTV (horizontal bars in experimental recordings present exposure periods of defined pharmacological agents; n, number of individual experiments; ns, not significant; time scale bars in all individual panels; Mann-Whitney U test).