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. Author manuscript; available in PMC: 2021 Oct 18.

Published in final edited form as: Methods Mol Biol. 2016;1365:25–62. doi: 10.1007/978-1-4939-3124-8_2

Table 1:

Yeast tagging cassette vectors

Plasmid family	Tag position	Promoter	Tags	Markers
pFA6a ¹	C terminal	endogenous	GFP(S65T) 3xHA 13xMyc GST	TRP1 kanMX6 HIS3MX6
pFA6a-PGAL1 ¹	N terminal or internal	GAL1	GFP(S65T) 3xHA GST	TRP1 kanMX6 HIS3MX6
pUR ²	C terminal	endogenous	DsRed	HIS3 URA3(K.l.)
pYM ³	C terminal	endogenous	yEGFP EGFP EBFP ECFP EYFP DsRed, DsRedI RedStar, RedStar2 eqFP611 FlAsH 1xHA, 3xHA, 6xHA 3xMyc, 9xMyc 1xMyc+7xHis TAP Protein A	kanMX4 hphNT1 natNT2 HIS3MX6 klTRP1
pKT ⁴	C terminal	endogenous	yEGFP yECFP yEVenus yECitrine yESapphire yEmCFP⁵ yEmCitrine tdimer2⁶ yECitrine+3xHA yECitrine+13xMyc yECFP+3xHA yECFP+13xMyc	KanMX SpHIS5 CaURA3
pOM ⁷	N terminal or internal	endogenous⁸	yEGFP 6xHA 9xMyc Protein A TEV-ProteinA TEV-GST-6xHis TEV-ProteinA-7xHis	kanMX6 URA3(K.l.) LEU2(K.l.)
pCY ⁹	C-terminal	endogenous	Cerulean yECFP yEmCFP yEGFP Venus yEVenus yECitrine yEmCitrine mCherry mEos2 yEc-MYC yEHA yEFlAsH	HygromycinB Zeocin

¹

(Longtine et al., 1998)

²

(Rodrigues et al., 2001)

³

(Janke et al., 2004)

⁴

(Sheff & Thorn, 2004)

⁵

monomeric version

⁶

tandem dimer of DsRed

⁷

(Gauss et al., 2005)

⁸

After Cre-mediated removal of auxotrophic marker

⁹

(Young et al., 2012)