Skip to main content
. Author manuscript; available in PMC: 2021 Oct 18.
Published in final edited form as: Methods Mol Biol. 2016;1365:25–62. doi: 10.1007/978-1-4939-3124-8_2

Table 5:

Conditions for staining yeast mitochondria with vital dyes

Dye [Stock]1 Staining conditions
[Dye] Incubation
DiOC6(Riezman et al., 1983)2 17.5 mM 17.5 nM 15 min RT
DASPMI3 1 mg/ml in ethanol 10–100 μg/ml 30 min RT
Rhodamine 1234 10 mg/ml in DMSO5 5–10 μg/ml 15–30 min RT
MitoTracker6 1 mM in DMSO5 100 nM 30 min RT
DAPI 1 mg/ml in H2O 0.1 μg/ml 15 min RT
1

All stock solutions should be stored in the dark.

4

(Skowronek et al., 1990); Broad emission range; not recommended for green/red double-label studies.

5

DMSO can be toxic and inhibits partitioning of the dye into the aqueous environment of the cells. If DMSO is the solvent, use a stock concentration that is at least 100X.