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. Author manuscript; available in PMC: 2021 Oct 18.
Published in final edited form as: Methods Mol Biol. 2016;1365:25–62. doi: 10.1007/978-1-4939-3124-8_2

Table 6:

Suggested imaging conditions for methods described in this chapter

FP/Dye Excitation/Emission Light source Exposure time2
Cit1-GFP 488/507 LED 470 nm (100%) or Metal-Halide Lamp + standard GFP Filter 100 ms
Tom70-mCherry 587/610 Metal-halide lamp + standard DsRed filter 200 ms
MitoTracker Red (100 nM for 30 min) 581/644 Metal-halide lamp + standard DsRed filter 200 ms
DiOC6 482/504 LED 470 nm (100%) 50 ms
roGFP (Oxidized) 365/507 LED 365 nm (25%) 100 ms
roGFP (Reduced) 470/507 LED 470 nm (100%) 100 ms
DHE (40 μM for 30 min) 485/530 Metal-halide lamp + standard DsRed filter 100 ms
Abp140-GFP 488/507 LED 470 nm (100%) or Metal-Halide Lamp + standard GFP Filter 300 ms
Abp1-mCherry 587/610 Metal-halide lamp + standard DsRed filter 200 ms
Cit1-mCherry 587/610 Metal-halide lamp + standard DsRed filter 200 ms
1

These are recommended concentrations, and can be adjusted as needed

2

These are recommended exposure times and can be adjusted as needed