KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
Aeromonas veronii ZOR0001	Karen Guillemin lab; Stephens et al., 2016	BioProject Accession PRJNA205572
Aeromonas caviae ZOR0002	Karen Guillemin lab; Stephens et al., 2016	BioProject Accession PRJNA205572
Aeromonas veronii ZOR0001, ΔspdE	This study	N/A
Aeromonas veronii ZOR0001, ΔcheA	This study	N/A
Aeromonas veronii ZOR0001, ΔspdE_comp	This study	N/A
Aeromonas veronii ZOR0001, ΔspdE_compevol	This study	N/A
Aeromonas veronii ZOR0001, ΔspdE_compAer02	This study	N/A
Aeromonas veronii ZOR0001, WTanc_OE	This study	N/A
Aeromonas veronii ZOR0001, dTomato	This study	N/A
Aeromonas veronii ZOR0001, superfolder GFP	This study	N/A
Escherichia coli BL21 DE3	New England Biolabs	Cat# C2527H
Escherichia coli SM10	Karen Guillemin lab	N/A
Chemicals, Peptides, and Recombinant Proteins
Ni-NTA resin	Qiagen	Cat# 30210
SYPRO Orange dye	Thermo Fisher	Cat# S6650
Collagenase P	Sigma	Cat# 11213857001
Critical Commercial Assays
Nextera XT Library Prep Kit	Illumina	Cat# FC-131–1096
Promega Wizard genomic DNA purification kit	Thermo Fisher	Cat# PR-A1120
Quant-iT dsDNA HS kit	Thermo Fisher	Cat# Q32851
Deposited Data
Raw sequence reads	This study	BioProject Accession PRJNA699275 (https://www.ncbi.nlm.nih.gov/sra/PRJNA699275)
A02 SpdEpp protein structure	This study	PDB ID: 7K5N
Experimental Models: Organisms/Strains
AB x Tu wild type zebrafish, Danio rerio	Univ. or Oregon Zebrafish facility	N/A
Myd88 mutant zebrafish, myd88−/−, Danio rerio	Karen Guillemin lab; Burns et al., 2017	N/A
Recombinant DNA
Plasmid: Aer01 spdEpp pBH	This study	N/A
Plasmid: Aer02 spdEpp pBH	This study	N/A
Plasmid: Aer01 spdEpp W140A pBH	This study	N/A
Plasmid: Aer01 spdEpp Y157A pBH	This study	N/A
Plasmid: Aer01 spdEpp Y211A pBH	This study	N/A
Plasmid: pAX1 allelic exchange vector	Karen Guillemin lab; Wiles et al., 2018	N/A
Software and Algorithms
breseq	Deatherage et al., 2014	https://barricklab.org/twiki/bin/view/Lab/ToolsBacterialGenomeResequencing
Phenix mr_rosetta	DiMaio et al., 2011	https://www.phenixonline.org/documentation/reference/mr_rosetta.html
particle tracking software	Parthasarathy lab Univ. of Oregon; GitHub	https://github.com/rplab/TrackingGUI_and_Localization_Public
Clustal Omega	McWilliam et al., 2013; Sievers et al., 2011	https://www.ebi.ac.uk/Tools/msa/clustalo/
BoxShade	N/A	https://embnet.vitalit.ch/software/BOX_form.html
HKL2000 suite	Otwinowski and Minor, 1997	https://hklxray.com/hkl-2000
Phenix 1.16.3549	Adams et al., 2010	https://www.phenixonline.org/
PyMOL 2.4.0	Schrödinger, LLC	https://pymol.org/2/
Other
Detailed protocol for genetically modifying Aeromonas	figshare.com	https://doi.org/10.6084/m9.figshare.7040258.v1