Fig. 1.

Analysis of cell viability upon treatment with single chemicals in hiPSC-derived NSCs undergoing differentiation. (A) NSCs were differentiated for 7 DIV and then treated for 14d with different nominal concentrations (as indicated) of: BDE47 (B), EtOH (C), Vincl (D) and TCDD (E), in comparison to solvent control (0.1 % DMSO, Ctr) at the respective time point. After 14d (i.e., 21 DIV), resazurin test was performed. All samples were normalised to solvent control (0.1 % DMSO, Ctr) at the respective time point. Chemicals were tested considering 6 internal replicates for each concentration (3-4 independent experimental replicates). Normalised values were imported into GraphPad Prism, where a non-linear fit (sigmoidal dose-response (variable slope)) was performed to calculate the inhibitory concentration (IC) values reported in (B-E).