Fig. 13.

Gastruloid quantification following ibuprofen exposure. Quantification of morphology of mouse gastruloids (a), and human gastruloids (b) including Area (top), Circularity (middle) and 1/Aspect Ratio (bottom). Dot colors indicate experimental replicates, and boxplots indicate spread of the data. Significant differences between DMSO (vehicle control) and ibuprofen (IB) treatment conditions are indicated in the plots (One-way ANOVA, Tukey’s post-hoc test, ɑ = 0.05indicated by asterisks (see Materials and Methods for thresholds)).