Table 1.
Administered compounds and their respective treatment concentrations.
| Compound | Teratogenicity classification | Treatment Concentrations [X1, X2, X3] (mouse) | Treatment Concentrations [X1, X2, (X3)] (human) |
|---|---|---|---|
| All-trans retinoic acid | Positive | 0.4 nM, 33 nM, 100 nM | 0.4 nM, 33 nM |
| Valproic Acid | Positive | 4 μM, 333 μM, 1000 μM | 4 μM, 333 μM |
| Bosentan | Positive | 4 μM, 8 μM, 18 μM | 4 μM, 18 μM |
| Thalidomide | Positive | 0.4 μM, 11 μM, 100 μM | 0.4 μM, 100 μM |
| Phenytoina | Positive | 1 μM, 5 μM, 20 μM | 20 μM |
| Ibuprofen | Positive | 63 μM, 120 μM, 250 μM | 63 μM, 250 μM |
| Penicillin G | Negative | 63 μM, 1 mM, 2 mM | 63 μM, 1 mM, 2 mM |
a
Phenytoin was soluble in DMSO at 200 mM but precipitates when mixed with PBS+/+, N2B27 or E6 medium at concentrations higher than 20 μM. Classification based on EMA ICH S5 (R3) guidelines on reproductive toxicology [5].