Fig 4. ORFV113 enhances p38 phosphorylation in infected cells.
(A) OFTu cells were mock-infected or infected with OV-IA82 (WT), OV-IA82-Δ113 (Δ113), OV-IA82-RV113HA (HA) and OV-IA82-RV113 (UN) (MOI:10) for 1, 3 and 5 h p.i. Total cell protein extracts were resolved by SDS-PAGE, blotted and incubated with antibodies against phos-p38 and total p38. (B) Densitometric analysis of bands corresponding to phosphorylated p38. Densitometry of phos-p38 bands were normalized to the loading control total p38. Results are average of three independent experiments. Error bars represent mean±SD. Statistical analysis was performed using one-way ANOVA with post hoc Tukey test for multiple comparisons (*,#, P<0.05; **, P<0.01; NS: non-significant). * and # denote statistical significance compared to mock and WT, respectively. Statistical comparison of a treatment to WT is shown with a line drawn over the respective treatment bar.