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. 2021 Oct 6;17(10):e1009742. doi: 10.1371/journal.ppat.1009742

Fig 5. Increased Ki67 expression indicates enhanced turnover and delayed regeneration of DCs and monocytes.

Fig 5

(A) Ki67 expression in DC and monocyte subsets was analyzed by intracellular staining and flow cytometry in a subgroup of patients and controls. Healthy donors (H, black, n = 12), hospitalized COVID-19 negative patients (white symbols, n = 4), acute COVID-19 patients with mild/moderate (M, red, n = 9), severe (S, blue, n = 12) disease and recovered patients (n = 11). (A, C) Percentages of Ki67+ cells in each subset are shown (Kruskal-Wallis test with Dunn’s correction, * or # p<0.05). (B) Representative histogram of Ki67 signal in DC3 in one patient with moderate COVID-19 at different time points after diagnosis. (D) Representative results of Ki67 expression in mo 1 in one healthy, one moderate and one severe patient shown as dot plots. (E) Log2 fold changes of median MFI values of CD86, PD-L1 and HLADR in Ki67+ versus Ki67– cells within the indicated populations are shown in the heatmaps indicated by the color scale. (F) Representative results of Ki67, CD86 and PD-L1 expression in DC3 of a healthy control, and 2 COVID-19 patients with moderate and severe disease are shown.