FIG. 3.

TAF_II250 HAT mutant retains TBP and TAF_II150 binding. HA-tagged WT and HAT mutant TAF_II250 proteins were immunopurified from recombinant baculovirus-infected Sf9 cell extracts and immobilized on anti-HA antibody (Ab) bound to PAS. The PAS-bound proteins, as indicated, were incubated with bacterial or Sf9 extracts overexpressing His-tagged TBP (A) or FLAG-tagged TAF_II150 (B), respectively. The resulting dimers were dissociated from the PAS beads and analyzed by SDS-PAGE followed by silver staining and Western blot analysis using anti-TBP or anti-FLAG antibody as indicated. Positions of size markers are indicated in kilodaltons at the left.