FIG. 4.
Transcriptional requirement for TAFII250 HAT activity is promoter dependent. ts13 cells, maintained at permissive (33.5°C) or nonpermissive (39.5°C) temperature, were transiently transfected with the control (ts13) or indicated TAFII250 (WT or HATmt) expression vector and the cyclin A-, cyclin D1-, or c-fos-luciferase (luc) reporter construct. Approximately 24 h posttransfection, transcriptional activity of each promoter construct in relative light units (rlu) was determined and normalized for transfection efficiency. Note that the transcriptional activity of the c-fos promoter was greater than that of the cyclin promoters, as illustrated by the larger scale on the y axis. Presented are data from one representative experiment; similar results have been observed repeatedly.