Fig. 3.

Evaluation of immunogenic cell death in CT26 cancer cells. A) A schematic of the primary hallmarks of ICD: calreticulin (CRT) surface expression and ATP and HMGB1 release. Translocation of CRT to the cell surface and ATP release occur in pre-apoptotic and dying cells, and occur over a time course of hours. HMGB1 release occurs in dying and dead cells and occurs over a time course of days. B) Cells were incubated with peptide or micelles for 24 hours and surface expression of CRT was measured via flow cytometry. C) Extracellular HMGB1 concentration was measured in cell supernatant via ELISA after 48 hour incubation with peptide or micelles. Significance is calculated via ANOVA and denoted in regards to CP. D) Extracellular ATP was measured in cell supernatant via luciferin reaction.