Tumor reduction with combinatorial ICB in 4T1 and CT26 tumors. A) Time line of tumor inoculation and treatment. B) 4T1 tumor growth in mice treated with (i) PBS, (ii) ICB, (iii) DMM, or (iv) DMM+ICB. Combinatorial treatment of DMM+ICB significantly reduced tumor growth compared to PBS (****p < 0.0001), ICB (****p < 0.0001), and DMM (****p < 0.0001). Significance was calculated via two-way ANOVA and is denoted in regards to PBS. C) CT26 tumor growth in mice treated with (i) PBS, (ii) ICB, (iii) DMM, or (iv) DMM+ICB for the first 14 days after treatment. Mice receiving ICB and DMM+ICB treatment had significantly smaller tumors than mice treated with PBS- (****p < 0.0001) or DMM- (****p < 0.0001). There was no significance (ns) between ICB and DMM+ICB mice. Significance was calculated via two-way ANOVA and is denoted in regards to PBS. (n = 6 mice/group)