Table 2.
The characteristics of the primary and secondary antibodies for the immunofluorescence are depicted. The immunofluorescence stains of GFAP and NeuN were performed twice (*) using different antibody batches
| Dilution | Host | Company (headquarter, state, country) |
Catalogue number | Experiment number* | |
|---|---|---|---|---|---|
| Primary antibodies | |||||
| GFAP | 1:400 | Rabbit |
Agilent Dako (Santa Clara, CA, USA) |
Z0334 | 1 |
| GFAP | 1:400 | Mouse |
BD Pharmingen Inc (San Diego, CA, USA) |
556,330 | 2 |
| NeuN | 1:500 | Mouse |
Millipore Sigma (Burlington MA, USA) |
MAB377 | 1 |
| NeuN | 1:500 | Rabbit |
Millipore Sigma (Burlington MA, USA) |
ABN78 | 2 |
| Secondary antibodies | |||||
| Alexa Fluor 488, anti-rabbit | 1:200 | Donkey |
Invitrogen, Thermo Fisher, Scientific (Waltham, MA, USA) |
A21206 | 1 |
| Alexa Fluor 488, anti-mouse | 1:200 | Donkey |
Invitrogen, Thermo Fisher, Scientific (Waltham, MA, USA) |
A21202 | 1 |
| Alexa Fluor 568, anti-rabbit | 1:200 | Donkey |
Invitrogen, Thermo Fisher, Scientific (Waltham, MA, USA) |
A10042 | 2 |
| Alexa Fluor 568, anti-mouse | 1:200 | Donkey |
Invitrogen, Thermo Fisher, Scientific (Waltham, MA, USA) |
A10037 | 2 |