Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 18;12(11):960. doi: 10.1038/s41419-021-04251-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a HCT116 and SW480 cells were treated with tunicamycin for the indicated time. Cell lysates were analyzed for the expression of PHLPP1, PHLPP2, ATF4 and eIF2α and β-actin using Western blot. The phosphorylation status of eIF2α was detected using the phospho-eIF2α (p-eIF2α) antibody. The relative expression levels of PHLPP1 and PHLPP2 were obtained by normalizing to β-actin. b The relative expression of LC3B (gene name MAP1LC3B), BECN1 (beclin1), ATG12, PHLPP1, and PHLPP2 mRNA was determined using RT-qPCR following the treatment with tunicamycin for 24 h in HCT116 and SW480 cells. Data represent the mean ± SD (¶p < 0.0001, & p < 0.001 and *p < 0.05).