Figure 4.

425 nm visible light inhibits cell-associated SARS-CoV-2 infection and replication on Vero E6 cells (MOI 0.01). Vero E6 cells infected with SARS-CoV-2 at a MOI of 0.01 were exposed to the indicated doses of 425 nm light at 1-h post-infection. (A) SARS-CoV-2 samples were harvested for TCID₅₀ assays at 24- and 48-h post-infection (red and blue circles, respectively). The limit of detection (LOD) is indicated by the dashed line. (B) Percent reduction in SARS-CoV-2 virus was calculated at 24- and 48-h post-infection (red and blue lines, respectively). Cell viability was evaluated on Vero E6 cells that were not infected with virus and is represented as % cytotoxicity (black triangles/line). Data are represented as the mean of four independent measurements. Statistical analysis was executed using a Mann–Whitney test wherein * is p < 0.05 when compared to the 0 J/cm² control.