TABLE 2.
Reactivities of DR strains and prototype strains by ITSD-E and probe hybridization assay, VP1-2A sequence type, sequence at neurovirulence markers 472 and 2034, MHT, and neurovirulence score in transgenic mice
| Strain | ITSD-E result | SL strain/NSL strain ELISA reaction ratio | Probe hybridization result | VP1-2A sequence type | Result with the following Neurovirulence markera:
|
MHT (days) | Neurovirulence scoreb | |
|---|---|---|---|---|---|---|---|---|
| 5′ NC (472) | VP3 (2034) | |||||||
| Saukett | NSL | 0.2 | NSL | NSL | U | Phe | ||
| Sabin 3 | SL | 5.1c | SL | SL | C | Ser | 14 | Attenuated |
| Leon/37 | NSL | 0.2 | NSL | NSL | U | Phe | 5.66 | High |
| 81-16685 | DR | 0.9 | SL | SL | C | Ser | 14 | Attenuated |
| 88-8877 | DR | 0.9 | SL | SL | C | Phe | 6.66 | High |
| 87-10657 | DR | 1.1 | SL | SL | C | Phe | 10.16 | Intermediate |
| 85-11064 | DR | 0.9 | SL | SL | C | Ser | 7.5 | Intermediate |
| 85-1470 | DR | 0.5 | SL | SL | C | Ser | 8.16 | Intermediate |
| 82-21507 | DR | 1.0 | SL | SL | C | Ser | 13.66 | Attenuated |
| 88-8058 | DR | 0.6 | SL | SL | C | Phe | 14 | Attenuated |
a
According to Almond et al. (3).
b
Neurovirulence in transgenic mice with the human poliovirus receptor.
c
Average ratio for 10 Sabin serotype 3 strains isolated from healthy OPV-vaccinated persons and the prototype Sabin strain.