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. 2021 Oct 18;11:20624. doi: 10.1038/s41598-021-98977-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of amino acid sequences and predicted structures of the single-domain V_HH antibodies Z18 and Z26. (A) Amino acid sequences of Z18 and Z26. CDR regions are indicated and amino acids that are not identical are indicated by asterisks. Residues are numbered according to the IMGT numbering scheme³⁹. (B) Homology models of Z18 and Z26. The CDR-H3 and mutational positions are shown in orange and magenta, respectively. Protein images were generated with UCSF Chimera⁴⁰.