Table 1.

Thermal stabilities and binding affinities of Z18 mutants and Z26.

	Tm (°C)	kon (1/M·S)	koff (1/s)	KD (M)
Z18	58.6 ± 0.1	7.74 × 105	0.259	3.34 × 10–7
Z18-V2L	57.8 ± 0.4	7.54 × 105	0.276	3.66 × 10–7
Z18-G32D	57.8 ± 0.4	6.96 × 105	0.120	1.72 × 10–7
Z18-R50L	60.9 ± 0.4	6.89 × 105	0.284	4.12 × 10–7
Z18-I56V	55.1 ± 0.4	7.41 × 105	0.220	2.96 × 10–7
Z18-T61L	55.5 ± 0.7	4.33 × 106	0.037	8.63 × 10–9
Z18-G78A	66.8 ± 0.3	1.55 × 105	1.101	7.11 × 10–6
Z18-N85S	56.2 ± 0.9	6.92 × 105	0.238	3.44 × 10–7
Z18-Y114F	57.8 ± 0.8	7.45 × 106	0.096	1.29 × 10–8
Z18-S133R	59.2 ± 0.6	1.16 × 106	0.078	6.72 × 10–8
Z18-R50L/G78A/S133R	70.9 ± 0.1	1.76 × 106	0.785	4.47 × 10–7
Z18-G32D/R50L/G78A/S133R	70.6 ± 0.2	5.16 × 105	0.412	7.97 × 10–7
Z26	64.8 ± 0.4	1.51 × 106	8.16 × 10–4	5.40 × 10–10