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. 2021 Oct 18;12:6072. doi: 10.1038/s41467-021-26346-0

Fig. 5. Single-cell RNA sequencing of the iridocorneal angle region of control and glaucomatous Angpt1ΔNC eyes.

Fig. 5

A Single-cell RNA sequencing was performed on pooled eyes from 6-week-old control and Angpt1ΔNC mice. Samples were integrated using CCA and clustered using the Seurat package in R before plotting using UMAP. B Overlaying samples from control and Angpt1ΔNC eyes shows that similar cell populations were present in each sample. C Angpt1 was detected in only a small number of cells isolated from Angpt1ΔNC eyes, indicating that the majority of Angpt1-expressing cells are derived from the neural crest. D Angpt1 expressing cells in WT mice were concentrated in clusters 11, 15, and 16. E Dendrogram and representative marker gene expression in each cluster identified. RPE, retinal pigment epithelium, LSC, limbal stem cell, conj. ep, conjunctival epithelium, lim. ep., limbal epithelium, cor. ep., corneal epithelium, mela, melanocyte, cor. str., corneal stroma, TM, trabecular meshwork, PE/NPE, ciliary (non) pigmented epithelium.