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. 2021 Oct 18;11:20627. doi: 10.1038/s41598-021-99974-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Heat map of compound library screen including 700 compounds. 10 selected compound classes are highlighted as indicated. Colors indicate grades of effective nematocyst discharge derived from prey capture efficiency as recorded in the primary screen. Grey, grade 3 (prey capture as in control); yellow, grade 2; orange, grade 1; red, grade 0 (full prey capture inhibition). The applied grades are further detailed in methods. Compound classes are indicated with boxes and numbers. Detailed data are given in Supplementary Table S1 online. (b) Average nematocyst discharge grade for tested compound classes applying levels 0–3 obtained from the primary screen (no influence on prey capture equals 3). 1 = pyrazoles, 2 = triazenes, 3 = [2.2]paracyclophanes (orange), 4 = coumarins, 5 = sugars, 6 = (per)fluorinated compounds, 7 = piperazines, 8 = steroids, 9 = azo-compounds, 10 = indole derivatives. NA (not assigned) = compound with no assignment to a certain compound class (grey).