Table 2.

Novel genic SSR genetic diversity values in 25 Millet individuals: allele ranges, observed heterozygosity (Ho), expected heterozygosity (He), and (PIC) values of 24 loci.

SSR loci	Repeat motifs	Allele ranges (bp)	Ho	He	PIC	Na	SPI
LtM 10	(CCA)7	125–144	0.12	0.36	0.34	3	3.04
LtM 332	(GGAAG)5	145–170	0.44	0.86	0.73	5	4.86
LtM 237	(AGC)8	158–179	0.55	0.85	0.61	5	5.83
LtM 3287	(GCCTCC)4	145–170	0.62	0.92	0.9	5	2.25
LtM 332	(GGAAG)5	135–160	0.69	0.68	0.66	5	0.54
LtM 7	(GCG)6	146–162	0.43	0.79	0.54	6	9.76
LtM 83	(GGACA)4	147–161	0.49	0.76	0.72	6	0.85
LtM 1207	(TGAGCT)4	105–153	0.56	0.75	0.72	6	3.05
LtM 340	(TCG)8	138–151	0.61	0.8	0.56	6	6.93
LtM 3383	(CCAAT)4	140–162	0.47	0.74	0.49	7	5.2
LtM 871	(CTAG)6	160–192	0.48	0.76	0.54	7	3.4
LtM25	(CCTC)5	120–150	0.31	0.32	0.11	8	3.24
LtM 1808	(CAG)8	118–130	0.63	0.87	0.65	8	5.22
LtM 115	(GCT)5	110–167	0.41	0.81	0.58	9	4.32
LtM 429	(GATG)5	157–179	0.46	0.81	0.77	9	1.02
LtM 32	(CAGAG)4	170–240	0.56	0.79	0.53	9	7.08
LtM 259	(GCT)8	140–159	0.32	0.63	0.34	10	3.8
LtM 83	(GGACA)4	175–195	0.29	0.77	0.52	11	10.2
LtM 1060	(CATGGC)4	155–166	0.39	0.58	0.53	11	2.16
LtM 109	(GGC)7	100–178	0.4	0.64	0.54	11	2.9
LtM 103	(TTGGA)4	148–162	0.27	0.66	0.59	12	0.88
LtM 1903	(ATCTC)4	136–150	0.88	0.66	0.41	13	5.04
LtM 60	(AC)10	159–171	0.55	0.84	0.6	14	5.94
LtM 28	(GCT)7	120–150	0.57	0.88	0.68	15	4.77
LtM 38	(CTG)7	130–168	0.91	0.69	0.61	16	3.1
Mean			0.49	0.72	0.57	8.68	4.21

The observed heterozygosity (Ho) ranged from 0.12 to 0.91, with an average of 0.44, whereas the expected heterozygosity (He) ranged from 0.32 to 0.92. (Mean: 0.70).