Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 18;11:20583. doi: 10.1038/s41598-021-99951-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Characterization of the immuno-functionalized PDMS membrane filter for tumor cell enrichment. (a) A schematic showing the tumor cell enrichment process. Following the immuno-functionalization of the membrane filter, whole blood spiked with MDA-MB-231 tumor cells is driven through the filter to capture nucleated cells on the filter and discard anucleated cells. The mechanically retained tumor cells are then released from the membrane filter under reverse flow. (b) Measured capture rates of the MDA-MB-231 tumor cells and WBCs during filtration (n = 3). (c) Measured release rates of the (left axis) MDA-MB-231 tumor cells and (right axis) WBCs under different reverse flow rates (n = 3). (d) Fluorescence microscope images of the PDMS membrane filter (left) right after the filtration of the blood sample and (right) following the release of cells under reverse flow. The images show that the tumor cells were succesfully released from the filter while most of the immunocaptured WBCs were retained on the filter with the reverse flow. (e) Calculated average enrichment ratio for the spiked tumor cells as a function of different reverse flow rates.