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The accumulation of pathological αSyn in various brain regions at different time points. Representative images of αSyn inclusions in different brain areas at each time point in the PFF-injected side. Immunofluorescence analysis of αSyn (green) was performed in the substantia nigra (SN), somatosensory cortex, piriform cortex, amygdala, and hippocampus of PFF-injected mice at different inoculation time. Scale bar, 50 μm.
