Figure 6.

The temporal change of microglial and astrocytic activation in the striatum. (A,B) DAB images (A) and its OD (B) showed that ionized calcium-binding adaptor molecule 1- (Iba1-) positive microglial expression increased at 7 dpi and decreased with time in the PFF-injected (left column) and noninjected striatums (right column) (χ² = 71.86, p < 0.0001, df = 13). (C,D) DAB images (C) and its OD (D) showed that glial fibrillary acidic protein- (GFAP-) positive astrocytic expression in PFF injected increased at 7 dpi and decreased with time (χ² = 94.29, p < 0.0001, df = 13). Bars indicate SEM, n = 3–4/time point. Statistical significance was measured with a comparison of 0 dpi and each time point by post hoc analysis (the Kruskal–Wallis test, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001). Scale bar, 500 μm.