Figure 8.

Body weight and behavioral changes in PFF-injected mouse. (A) Time-dependent changes in body weight did not show the difference between PFF-injected mice and PBS-injected mice (p > 0.05). (B–G) The results of the rotarod and wire hang test at each time point. The wire hang test showed significant motor deficits from 90 days, whereas the rotarod test did not show. The results were obtained from the same mice group, respectively, at baseline and at the time of sacrifice. Six to eight mice per group (mice with the same dpi at sacrifice) were used for each experiment. (H,I) Hind limb clasping test. (H) Representative images of mice exhibiting the different scores of hind limb clasping. (I) Clasping score increased significantly with time compared to baseline. For the clasping score analysis, six to eight mice were used at each time point for this analysis. For the PBS group, five mice were used at 90 dpi. Data are presented as mean ± SEM (Wilcoxon signed-rank test, ^*p < 0.05, ^**p < 0.01).