FIGURE 1.
Characterization of mesenchymal stromal cells (MSC) obtained from young and old donors. (A) Surface marker expression by MSC isolated from young donors (30 and 45 years old) and old donors (60 and 80 years old) by flow cytometry. MSC were positive for CD73, CD90, and CD105 but negative for CD34 and CD45. (B) Flow cytometry plots of surface marker expression by young MSC. (C) Flow cytometry plots of surface marker expression by old MSC. (D) Multilineage differentiation analysis of MSC from young donors. (E) Multilineage differentiation analysis of MSC from old donors. Cells were cultured under osteogenic, chondrogenic, and adipogenic differentiation media for 21 days. DMEM: cells before differentiation (day 0), OSTEO, CHONDRO, and ADIPO: cells differentiated at day 21. Scale bars, 100 μm.