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. 2021 Oct 12;12:100147. doi: 10.1016/j.mtbio.2021.100147

Fig. 3.

Fig. 3

Effects of the Mg-coated Ti6Al4V scaffolds on the proliferation, migration, and invasion of 143B and HOS cells. (A) Schematic diagram showing OS cell coculture with the scaffolds. (B) CCK-8 assay analysis of 143B and HOS cell proliferation after 0, 24, 48, 72, and 96 h. (C) Live/dead viabilities of 143B and HOS cells cocultured for 48 h (live cells are stained green, and dead cells are stained red). (D) Images and (E) colony numbers were obtained from the plate cloning assay after 14 days. n = 3; ∗∗p < 0.01. (F) Images of wound healing and (G) invasion assays with 143B and HOS cells cocultured in the different groups. (H) The rates of wound repair and (I) numbers of migrated cells were analyzed. n = 3; ∗p < 0.05; ∗∗p < 0.01.