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. 2021 Oct 12;12:100147. doi: 10.1016/j.mtbio.2021.100147

Fig. 5.

Fig. 5

The Mg-coated Ti6Al4V scaffolds induced an increased level of autophagy in apoptotic 143B and HOS cells. (A) Fluorescent inverted microscopy analysis of LC3 puncta in 143B and HOS cells cocultured for 48 h (red; the cells were counterstained with DAPI to show nuclei in blue). (B) Transmission electron microscopy of autophagosomes in 143B and HOS cells. (C) Fluorescent inverted microscopy of GFP-mCherry-LC3 adenovirus-transfected 143B and HOS cells after 48 h. (D) The relative LC3B and p62 expression levels were detected by Western blot analysis and determined by greyscale analysis. n = 3; ∗p < 0.05; ∗∗p < 0.01.