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. 2021 Oct 12;12:100147. doi: 10.1016/j.mtbio.2021.100147

Fig. 6.

Fig. 6

The inhibition of autophagy alleviated 143B and HOS cell apoptosis induced by the Mg-coated Ti6Al4V scaffolds. (A) 143B and HOS cells were cocultured with the Mg-coated Ti6Al4V scaffolds (MgB) in the presence or absence of 10 mM 3-MA or 20 μM CQ; pM and inhibitor groups without scaffolds were established as controls. LC3 puncta formation was captured by fluorescence inverted microscopy. (B) TEM images of autophagosomes in 143B and HOS cells. (C) Autophagic flux detection in 143B and HOS cells. (D) Flow cytometry analysis of 143B and HOS cell apoptosis after coculture. n = 3; ∗p < 0.05; ∗∗p < 0.01.