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Anti-OS effects of the Mg-coated Ti6Al4V scaffolds in vivo. (A) Tumor growth in tumor-bearing mice treated with different extracts. (B) Images and calculated volumes of harvested tumors after 30 days. n = 3; ∗∗p < 0.01. (C) TUNEL staining (apoptotic cells shown in red) and histological IF staining of Bcl-2, p62, LC3, p-AMPK, p-mTOR, and p-ULK1 in tumor sections (red indicates positive expression; the nuclei were stained blue by DAPI). (D) HE staining of tumor tissues.
