FIGURE 1.

Screening the effective components in zedoary turmeric oil which inhibit the activity of HSCs. (A) LX-2 cells were treated with Zedoary turmeric oil (0.2 μL/ml) and Curcumin, Curcumol, Germarcone (GER), Curzerene, Curcumenol, Isocurcumenol, and Curdione (CUR) (40 μM) for 25 h, and then were stimulated by TGF- β (10 ng/ml) for 24 h. The expression of collagen I was detected by western blot, and GAPDH was used as the loading control. (B) L02 cells were treated with CUR for 24 h, then the cell viability was detected by CCK-8 (n = 3). (C) LX-2 cells were treated with CUR for 24 h, then the cell viability was detected by CCK-8 (n = 3). (D) L02 cells were treated with GER for 24 h, then the cell viability was detected by CCK-8 (n = 3). (E) LX-2 cells were treated with GER for 24 h, then the cell viability was detected by CCK-8 (n = 3). All the results were compared with control group. All data are presented as means ± SEM. ***p < 0.001 vs. the control group.