FIGURE 4.

GER protects the hepatocyte by inhibiting the release of mitochondrial ROS in vitro. (A) L02 cells were treated with APAP (20 mM) for 12 h, and then were treated with GER or NAC for 24 h, then the cell viability was detected by CCK-8 (n = 3). (B) L02 cells were treated with H₂O₂ (600 μM) for 12 h, and then were treated with GER or NAC for 24 h, then the cell viability was detected by CCK-8 (n = 3). (C) Quantitative analysis of different group mitochondrial ROS release (n = 3). (D) Flow cytometric analysis of the cell proportion of mitochondrial ROS in L02. The NAC was used as positive control, every group was treated with APAP except for control group. All data are presented as means ± SEM. ^### p < 0.001 vs. the control group. ^** p < 0.01, ^*** p < 0.001 vs. the APAP group.