TABLE 2.

Sequence of rat primers used in RT-PCR experiment.

Gene	Accession No.	Primer sequence	PCR Cycle no.	Annealing Tm (°C)
GRM1	NM_001114330.1	F: 5′-CCAGTGATGTTCTCCATACC-3′ R: 5′-CACTCTGGGTAGACTTGAGTG-3′	40	50
GRM5	NM_017012.1	F: 5′-CCCCAAACTCTCCAGTCT-3′ R: 5′-ATTTTTCACCTCGGGTTC-3′	40	49
β-actin	NM_031144.3	F: 5′-GGGAAATCGTGCGTGACATT-3′ R: 5′-CGGATGTCAACGTCACACTT-3′	40	55

GRM, glutamate metabotropic receptor; Tm, temperature; F, forward primer; R, reverse primer.