Table 1.
List of primers used for pyrosequencing.
| Gene | Primer | Sequence (5′-3′) | Length (bp) | Accession no. (Gene ID) | Annealing Tm (°C) |
|---|---|---|---|---|---|
| LINE-1 | Forward | TTTTGAGTTAGGTGTGGGATATA | 146 | NM_019079 (54596) | 50 |
| Reverse | [BTN] AAAATCAAAAAATTCCCTTTC | ||||
| Sequencing | AGTTAGGTGTGGGATATAG | ||||
| NCAM1 | Forward | TATTTTTGTGTTTTTTTGGGGGTTAGATTA | 154 | NM_181351 (4684) | 55 |
| Reverse | [BTN] CCCAACTATACAATCTTCTCTACTTCAT | ||||
| Sequencing | GGGGTTAGATTATTTTTTGAT | ||||
| NGF | Forward | Hs_NGF_01_PM PyroMark CpG assay (PM00002618) | 211 | NM_002506 (4803) | 58 |
| Reverse | |||||
| Sequencing |
LINE-1, Long interspersed element-1; NCAM1, Neural Cell Adhesion Molecule; NGF, Nerve Growth Factor.