Table 4.

Pyrosequencing results of NCAM1 showing percent change in methylation in ASD group, ANMR and AMR subgroups compared to healthy control group.

	NCAM1
	Control vs. ASD		Control vs. ANMR		Control vs. AMR
	Percent change	p-value	Percent change	p-value	Percent change	p-value
	in methylation		in methylation		in methylation
All CpG	−0.96***	0.0009	−0.96**	0.005	−0.97*	0.016
CpG site 1	−1.41***	<0.0001	−1.31***	0.0003	−1.57***	0.0002
CpG site 2	−0.43	0.16	−0.46	0.19	−0.37	0.34

ASD, autism spectrum disorders; AMR, autism mental regression; ANMR, autism non-mental regression; NCAM1, Neural Cell Adhesion Molecule.

^*P ≤ 0.05;

^**P < 0.01;

^***P < 0.001 with statistically significant changes highlighted in bold.