Table 5.

Pyrosequencing results of NGF showing percent change in methylation in ASD group, ANMR and AMR subgroups compared to healthy control group.

	NGF
	Control vs. ASD		Control vs. ANMR		Control vs. AMR
	Percent change	p-value	Percent change	p-value	Percent change	p-value
	in methylation		in methylation		in methylation
All CpG	−0.05	0.20	0.04	0.81	−0.28**	0.029
CpG site 1	−0.13	0.21	−0.07	0.61	−0.24	0.09
CpG site 2	0.04	0.83	0.11	0.35	−0.09	0.57
CpG site 3	−0.49***	0.0004	−0.51	0.0008***	−0.67**	0.001

ASD, autism spectrum disorders; AMR, autism mental regression; ANMR, autism non-mental regression; NGF, Nerve Growth Factor.

^*P ≤ 0.05;

^**P < 0.01;

^***P < 0.001 with statistically significant changes highlighted bold.