Figure 2.

HES1 level does not increase as NSCs transition from proliferation into quiescence

(A) Graphs showing luminescence expression of LUC2:HES1 NSCs as they transition from proliferation into quiescence from 3 independent experiments. Red vertical lines indicate the time at which proliferation media was replaced by quiescence media and horizontal dotted lines represent the luminescence thresholds defined as the median luminescence expression across proliferative and quiescence conditions per experiment, estimated by the median luminescence expression at each sampling interval (A.U. = arbitrary units).

(B) Graph showing the relative fold change of median luminescence expression between proliferative and quiescent conditions per experiment. Luminescence expression was estimated by the median luminescence expression at each sampling interval across proliferative and quiescent cell traces (error bars represent standard deviation, two-tailed paired ttest, ns = not significant).

(C) Schematic showing the different parameters tested for each peak from all cell traces. These include the maximum fold change of luminescence expression per peak relative to the threshold, the continuous time spent above the threshold for every peak and the area under the curve per peak.

(D–F) Graphs comparing the time spend above threshold per peak (D), the area under the curve (E) and the maximum fold change above threshold per peak (F) between proliferative and quiescent conditions per experiment (circles represent mean values and error bars represent standard deviation, two-tailed paired ttest, ns = not significant)