TABLE 1.

Cell cycle analysis.

	Cell cycle phase
	G1/G0	S	G2/M
3 WK CTRL	69.9%	7.7%	17.8%
4 WK CTRL	81.1%*	4.1%*	11.6%
6 WK CTRL	87.6%**	2.1%**	9.5%**
3 WK 3 mM Met	66.9%	9.1%	19.7%
4 WK 3 mM Met	79.8%	5.2%	12.5%
6 WK 3 mM Met	77.5%	4.5%#	16.1%
3 WK 6 mM Met	68.1%	9.1%	19.2%
4 WK 6 mM Met	82.0%	4.7%	10.9%
6 WK 6 mM Met	76.9%	4.7%#	16.2%#
3 WK 9 mM Met	64.0%	8.9%	21.2%
4 WK 9 mM Met	81.4%	4.5%	11.8%
6 WK 9 mM Met	76.9%	6.7%##	14.6%

This table shows the percentage of cells in each of the different phase of cell cycles after 3, 4 and 6 weeks of in vitro cultures, either in the absence (CTRL) or presence of metformin. For each condition (CTRL, 3 mM, 6 mM and 9 mM metformin), the symbol (*) indicates the statistical difference between 3 weeks of treatment (chosen as reference) and the other time periods. For each time point (3, 4 and 6 weeks), the symbol (#) indicates the difference between the CTRL and the other conditions. The symbols * or # and ** or ## correspond to p < 0.05 and p < 0.01, respectively. The values in bold indicate the most significant changes in S phase following metformin treatment.