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. 2021 Oct 6;46(6):250. doi: 10.3892/or.2021.8201

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Copyright: © Xie et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Transfection efficiency of MBD2 and EZH2 in colorectal tumor cells (SW480 and HCT116). qPCR screening of the most efficient EZH2 interference sequence in SW480 (A) and HCT116 (B) cells; qPCR was used to screen the most efficient MBD2 interference sequence in SW480 (C) and HCT116 (D) cells. (E) Western blot analysis was used to identify the efficiency of si-MBD2-001 and si-EZH2-2196 in SW480 and HCT116. *P<0.05, **P<0.01, ***P<0.001 vs. si-NC. MBD2, methyl-CpG binding domain protein 2; EZH2, enhancer of zeste homolog 2.