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. 2021 Oct 6;46(6):250. doi: 10.3892/or.2021.8201

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Copyright: © Xie et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 7. — Effects of MBD2 and EZH2 on cell cycle distribution of CRC SW480 and HCT116 cells. Flow cytometry was used to detect the cell cycle distribution following knockdown of MBD2, (A) EZH2, (B) MBD2 and EZH2 (C) and control group si-NC (D) in SW480 cells. Flow cytometry was used to detect the cell cycle distribution following knockdown of MBD2, (E) EZH2, (F) MBD2 and EZH2 (G) and control group si-NC (H) in HCT116 cells. Histogram showing the effects of the knockdown of MBD2 and EZH2 on the cell cycle distribution of SW480 (I) and HCT116 (K) cells. Statistical analysis was performed to analyze the effect of the knockdown of MBD2 and EZH2 separately and simultaneously on the S phase of the cell cycle in SW480 (J) and on the G0/G1 phase of the cell cycle in HCT116 (L) cells. **P<0.01, ***P<0.001 vs. si-NC. CRC, colorectal cancer; MBD2, methyl-CpG binding domain protein 2; EZH2, enhancer of zeste homolog 2.