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. 2021 Oct 6;46(6):250. doi: 10.3892/or.2021.8201

Table I.

SFRP primer sequence information for MSP experiments.

Gene name Primer sequence
SFRP1(U) F: 5′-GTTTTGTAGTTTTTGGAGTTAGTGTTGTGT-3′
R: 5′-CCTACGATCGAAAACGACGCGAACG-3′
SFRP2(U) F: 5′-TTTTGGGTTGGAGTTTTTTGGAGTTGTGT-3′
R: 5′-AACCCACTCTCTTCACTAAATACAACTCA-3′
SFRP4(U) F: 5′-GGGGGTGATGTTATTGTTTTTGTATTGAT-3′
R: 5′-CACCTCCCCTAACATAAACTCAAAACA-3′
SFRP5(U) F: 5′-GTAAGATTTGGTGTTGGGTGGGATGTTT-3′
R: 5′-AAAACTCCAACCCAAACCTCACCATACA-3′
SFRP1(M) F: 5′-TGTAGTTTTCGGAGTTAGTGTCGCGC-3′
R: 5′-CCTACGATCGAAAACGACGCGAACG-3′
SFRP2(M) F: 5′-GGGTCGGAGTTTTTCGGAGTTGCGC-3′
R: 5′-CCGCTCTCTTCGCTAAATACGACTCG-3′
SFRP4(M) F: 5′-GGGTGATGTTATCGTTTTTGTATCGAC-3′
R: 5′-CCTCCCCTAACGTAAACTCGAAACG-3′
SFRP5(M) F: 5′-AAGATTTGGCGTTGGGCGGGACGTTC-3′
R: 5′-ACTCCAACCCGAACCTCGCCGTACG-3′

MSP, methylation specific PCR; U, unmethylated; M, methylated; SFRP, secreted frizzled related protein.