Table I.
Gene name | Primer sequence |
---|---|
SFRP1(U) | F: 5′-GTTTTGTAGTTTTTGGAGTTAGTGTTGTGT-3′ |
R: 5′-CCTACGATCGAAAACGACGCGAACG-3′ | |
SFRP2(U) | F: 5′-TTTTGGGTTGGAGTTTTTTGGAGTTGTGT-3′ |
R: 5′-AACCCACTCTCTTCACTAAATACAACTCA-3′ | |
SFRP4(U) | F: 5′-GGGGGTGATGTTATTGTTTTTGTATTGAT-3′ |
R: 5′-CACCTCCCCTAACATAAACTCAAAACA-3′ | |
SFRP5(U) | F: 5′-GTAAGATTTGGTGTTGGGTGGGATGTTT-3′ |
R: 5′-AAAACTCCAACCCAAACCTCACCATACA-3′ | |
SFRP1(M) | F: 5′-TGTAGTTTTCGGAGTTAGTGTCGCGC-3′ |
R: 5′-CCTACGATCGAAAACGACGCGAACG-3′ | |
SFRP2(M) | F: 5′-GGGTCGGAGTTTTTCGGAGTTGCGC-3′ |
R: 5′-CCGCTCTCTTCGCTAAATACGACTCG-3′ | |
SFRP4(M) | F: 5′-GGGTGATGTTATCGTTTTTGTATCGAC-3′ |
R: 5′-CCTCCCCTAACGTAAACTCGAAACG-3′ | |
SFRP5(M) | F: 5′-AAGATTTGGCGTTGGGCGGGACGTTC-3′ |
R: 5′-ACTCCAACCCGAACCTCGCCGTACG-3′ |
MSP, methylation specific PCR; U, unmethylated; M, methylated; SFRP, secreted frizzled related protein.