FIG 2.

Primary osteoblasts express more α5β1-integrin than A549 cells, and the fibrillary presentation of detergent-insoluble Fn represents the prominent Fn conformation on pHOB. (A) Cells were detached with accutase and incubated with anti-α5 or anti-β1 antibody before flow-cytometric analysis. Data represent the means ± SD from 3 independent experiments. *, P < 0.05; **, P ≤ 0.01, unpaired t test. (B) Representative immunofluorescence microscopy images of Fn expression on A549 and pHOB using an antibody against Fn (white or red) and Hoechst 33342 for nucleic acid staining (blue). (C) After 3 days of growth, the DOC-insoluble and the DOC-soluble protein fractions of pHOB and A549 cells were harvested and analyzed by Western blotting. (D) Data are means ± SD from Western blot quantification of three independent experiments. *, P < 0.05; **, P ≤ 0.01, unpaired t test.