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. Author manuscript; available in PMC: 2021 Oct 19.
Published in final edited form as: Cell Chem Biol. 2021 Apr 8;28(5):648–661.e5. doi: 10.1016/j.chembiol.2021.03.011

Figure 4. Positioning of HT7 in dCas9 Governs the HT7 Fusion Protein Degradation by haloPROTACs.

Figure 4.

A) HP14 induces degradation of CT-dCas9HT7 fusion protein. The cell line stably overexpressing CT-dCas9HT7 was treated with HP14 (20 μM) and HP17 (20 μM) and lysed at indicated time points. Lysates were probed for HT7 and GAPDH.

B) The haloPROTAC HP3 (possessing a shorter linker) did not induce p50 degradation, in contrast to HP14.

C) Cells stably expressing NT-dCas9HT7 were treated with HP14 and lysed at indicated time points.

D) NFκB-TRAFTAC/HP14/TNF-alpha treated NT-dCas9HT cells were lysed and probed for p50 and tubulin levels.